DETAILED NOTES ON SB-431542

Subsequently, a two hundred-μl fixation buffer was utilized to fix the cells for 20 min. Right after washing the cells three times with precooled PBS, one× intracellular staining permeabilization clean buffer that contains 1% DAPI (blue) was placed on mark the nuclei with the cells. 10 minutes later, the cells had been washed three times with pr